human complementary dna (Sino Biological)

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Sino Biological human complementary dna

Influence of TLS polymerases on the TMZ-induced mutation spectra in the presence and absence <t>of</t> <t>hMGMT.</t> A, illustration of the experimental system. dsDNA substrates were incubated with 400 μM TMZ three times. When indicated, damaged templates were treated with hMGMT. The <t>DNA</t> was heated and reannealed with NGS primer and 10x excess competitor to sequester the top strand. Then the primer extension was started by adding yPol δ. After 30 min of incubation with yPol δ, the second polymerase (either yPol ζ, hPol κ, or hPol η) was added, and the reaction was continued for another 30 min. B – D, mutation spectra produced on the TMZ-damaged DNA in the presence of the indicated second polymerase without hMGMT treatment. E–G, influences of the second polymerase on the C>T mutations were expressed as a ratio of the mutation frequencies at individual sites. CpC>T and CpT>T mutations (SBS11), other mutations (Others), and all mutations (All) are plotted as separate groups. H – J, the same experiments as in B – D were carried out using the templates that were treated with hMGMT. K, influences of hMGMT on the C>T and C>A mutations that were produced in the presence of indicated second polymerases. For hPol η reactions, only C>T mutations were analyzed because this polymerase did not produce considerable C>A mutations. Mutation frequencies mapped on the templates are shown in . hMGMT, human methylguanine methyltransferase; hPol κ, human Pol κ; hPol η, human Pol η; NGS, next-generation sequencing; SBS11, substitution signature 11; TLS, translesion synthesis; TMZ, temozolomide; yPol δ, yeast Pol δ.

Human Complementary Dna, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mgmt+plasmid/Human+MGMT+Gene+ORF+cDNA+clone+expression+plasmid/pmc12556801-188-0-9
Average 91 stars, based on 3 article reviews

human complementary dna - by Bioz Stars, 2026-10

91/100 stars

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1) Product Images from "Biochemical reconstitution of temozolomide-induced mutational processes"

Article Title: Biochemical reconstitution of temozolomide-induced mutational processes

Journal: The Journal of Biological Chemistry

doi: 10.1016/j.jbc.2025.110676

Influence of TLS polymerases on the TMZ-induced mutation spectra in the presence and absence of hMGMT. A, illustration of the experimental system. dsDNA substrates were incubated with 400 μM TMZ three times. When indicated, damaged templates were treated with hMGMT. The DNA was heated and reannealed with NGS primer and 10x excess competitor to sequester the top strand. Then the primer extension was started by adding yPol δ. After 30 min of incubation with yPol δ, the second polymerase (either yPol ζ, hPol κ, or hPol η) was added, and the reaction was continued for another 30 min. B – D, mutation spectra produced on the TMZ-damaged DNA in the presence of the indicated second polymerase without hMGMT treatment. E–G, influences of the second polymerase on the C>T mutations were expressed as a ratio of the mutation frequencies at individual sites. CpC>T and CpT>T mutations (SBS11), other mutations (Others), and all mutations (All) are plotted as separate groups. H – J, the same experiments as in B – D were carried out using the templates that were treated with hMGMT. K, influences of hMGMT on the C>T and C>A mutations that were produced in the presence of indicated second polymerases. For hPol η reactions, only C>T mutations were analyzed because this polymerase did not produce considerable C>A mutations. Mutation frequencies mapped on the templates are shown in . hMGMT, human methylguanine methyltransferase; hPol κ, human Pol κ; hPol η, human Pol η; NGS, next-generation sequencing; SBS11, substitution signature 11; TLS, translesion synthesis; TMZ, temozolomide; yPol δ, yeast Pol δ.

Figure Legend Snippet: Influence of TLS polymerases on the TMZ-induced mutation spectra in the presence and absence of hMGMT. A, illustration of the experimental system. dsDNA substrates were incubated with 400 μM TMZ three times. When indicated, damaged templates were treated with hMGMT. The DNA was heated and reannealed with NGS primer and 10x excess competitor to sequester the top strand. Then the primer extension was started by adding yPol δ. After 30 min of incubation with yPol δ, the second polymerase (either yPol ζ, hPol κ, or hPol η) was added, and the reaction was continued for another 30 min. B – D, mutation spectra produced on the TMZ-damaged DNA in the presence of the indicated second polymerase without hMGMT treatment. E–G, influences of the second polymerase on the C>T mutations were expressed as a ratio of the mutation frequencies at individual sites. CpC>T and CpT>T mutations (SBS11), other mutations (Others), and all mutations (All) are plotted as separate groups. H – J, the same experiments as in B – D were carried out using the templates that were treated with hMGMT. K, influences of hMGMT on the C>T and C>A mutations that were produced in the presence of indicated second polymerases. For hPol η reactions, only C>T mutations were analyzed because this polymerase did not produce considerable C>A mutations. Mutation frequencies mapped on the templates are shown in . hMGMT, human methylguanine methyltransferase; hPol κ, human Pol κ; hPol η, human Pol η; NGS, next-generation sequencing; SBS11, substitution signature 11; TLS, translesion synthesis; TMZ, temozolomide; yPol δ, yeast Pol δ.

Techniques Used: Mutagenesis, Incubation, Produced, Next-Generation Sequencing, Translesion Synthesis

Expressing:

Article Title: A quantitative characterization of the heterogeneous response of glioblastoma U-87 MG cell line to temozolomide
Article Snippet: .. Eukaryotic expression plasmid for human MGMT tagged with GFPspark was purchased from SinoBiological (HG12077-ACG). .. Fugene HD transfection reagent (Promega; catalogue number: E231A) was used for transient transfections.

Article Title: A quantitative characterization of the heterogeneous response of glioblastoma U-87 MG cell line to temozolomide.
Article Snippet: .. Expression plasmid and transient transfection Eukaryotic expression plasmid for human MGMT tagged with GFPspark was purchased from SinoBiological (HG12077-ACG). .. Fugene HD transfection reagent (Promega; catalogue number: E231 A) was used for transient transfections.

Plasmid Preparation:

Amplification:

Article Title: Biochemical reconstitution of temozolomide-induced mutational processes
Article Snippet: .. Human complementary DNA of MGMT (hMGMT) was obtained from SinoBiological , amplified by PCR, and cloned into pET21a to express MGMT with a C-terminal His6-tag. .. After confirming the DNA sequence, the plasmid was introduced into Escherichia coli BLR(DE3) harboring the pCodonPlus plasmid (Agilent Technologies) for overexpression of the protein.

Polymerase Chain Reaction:

Clone Assay:

other:

Article Title: O-6-methylguanine DNA methyltransferase is a favorable biomarker with proliferation suppressive potential in Breast Cancer
Article Snippet: The pCMV empty vector and pCMV-MGMT plasmids were designed and provided by Sino Biological (Beijing, China).

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